SPAG5 antibody (AA 738-1193)

Catalog No. ABIN7602399

Product Details anti-SPAG5 Antibody

Target: SPAG5 (Sperm Associated Antigen 5 (SPAG5))

Binding Specificity: AA 738-1193

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPAG5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Purpose: Anti-Astrin/Deepest/SPAG5 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Astrin/Deepest/SPAG5 Antibody Picoband® (ABIN7602399). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Astrin/Deepest/SPAG5 recombinant protein (Position: E738-S1193).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chang, M.-S., Huang, C.-J., Chen, M.-L., Chen, S.-T., Fan, C.-C., Chu, J.-M., Lin, W.-C., Yang, Y.-C. Cloning and characterization of hMAP126, a new member of mitotic spindle-associated proteins. Biochem. Biophys. Res. Commun. 287: 116-121, 2001. 2. Cheng, T.-S., Hsiao, Y.-L., Lin, C.-C., Yu, C.-T. R, Hsu, C.-M., Chang, M.-S., Lee, C.-I., Huang, C.-Y. F., Howng, S.-L., Hong, Y.-R. Glycogen synthase kinase 3-beta interacts with and phosphorylates the spindle-associated protein astrin. J. Biol. Chem. 283: 2454-2464, 2008. 3. Du, J., Jablonski, S., Yen, T. J., Hannon, G. J. Astrin regulates Aurora-A localization. Biochem. Biophys. Res. Commun. 370: 213-219, 2008.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SPAG5

Target: SPAG5 (Sperm Associated Antigen 5 (SPAG5))

Alternative Name: SPAG5 (SPAG5 Products)

Background:

Synonyms: Mimecan, Osteoglycin, Ogn, Og

Tissue Specificity: Expressed in kidney.

Background: Sperm-associated antigen 5 is a protein that in humans is encoded by the SPAG5 gene. This gene encodes a protein associated with the mitotic spindle apparatus. The encoded protein may be involved in the functional and dynamic regulation of mitotic spindles.

Molecular Weight: 130 kDa, 150 kDa

Gene ID: 10615

UniProt: Q96R06

Pathways: M Phase