CD40 Ligand antibody (AA 76-249)

Catalog No. ABIN7602423

This Rabbit Polyclonal antibody specifically detects CD40 Ligand in WB, FACS and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CD40 Ligand antibody (AA 76-249) (ABIN7602423)

Target: CD40 Ligand (CD40LG)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD40 Ligand antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Product Details anti-CD40 Ligand Antibody

Binding Specificity: AA 76-249

Purpose: Anti-TRAP/CD40L/CD40LG Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TRAP/CD40L/CD40LG Antibody Picoband® (ABIN7602423). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TRAP/CD40L/CD40LG recombinant protein (Position: E76-H249).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Allen,R.C.,Armitage,R.J.,Conley,M.E.,Rosenblatt,H.,Jenkins,N.A.,Copeland,N.G.,Bedell,M.A.,Edelhoff,S.,Disteche,C.M.,Simoneaux,D.K.,Fanslow,W.C., Belmont,J.,Spriggs,M.K.:CD40 ligand gene defects responsible for X-linked hyper-IgM syndrome. Science 259: 990-993, 1993. 2. Cipollone,F.,Ferri,C.,Desideri,G.,Paloscia,L.,Materazzo,G.,Mascellanti,M.,Fazia,M.,Iezzi,A.,Cuccurullo,C.,Pini,B.,Bucci,M.,Santucci,A.,Cuccurullo,F., Mezzetti,A.:Preprocedural level of soluble CD40L is predictive of enhanced inflammatory response and restenosis after coronary angioplasty. Circulation 108: 2776-2782, 2003.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CD40 Ligand

Target: CD40 Ligand (CD40LG)

Alternative Name: CD40LG

Background:

Synonyms: CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5, CD154, CD40LG, CD40L, TNFSF5, TRAP

Tissue Specificity: Specifically expressed on activated CD4+ T-lymphocytes.

Background: CD40 ligand (CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb /IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells.CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.

Molecular Weight: 36 kDa

Gene ID: 959

UniProt: P29965

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints