Fibrillarin antibody (AA 77-138)

Catalog No. ABIN7602434

This anti-Fibrillarin antibody is a Rabbit Polyclonal antibody detecting Fibrillarin in WB, ELISA, IF, ICC and FACS. Suitable for Human and Monkey.

Quick Overview for Fibrillarin antibody (AA 77-138) (ABIN7602434)

Target: Fibrillarin (FBL)

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fibrillarin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-Fibrillarin Antibody

Binding Specificity: AA 77-138

Purpose: Anti-Fibrillarin/FBL Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Fibrillarin/FBL Antibody Picoband® (ABIN7602434). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Fibrillarin/FBL recombinant protein (Position: K77-N138).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Monkey
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Aris, J. P., Blobel, G. cDNA cloning and sequencing of human fibrillarin, a conserved nucleolar protein recognized by autoimmune antisera. Proc. Nat. Acad. Sci. 88: 931-935, 1991. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 7/9/2010. 3. Okano, Y., Steen, V. D., Medsger, T. A., Jr. Autoantibody to U3 nucleolar ribonucleoprotein (fibrillarin) in patients with systemic sclerosis. Arthritis Rheum. 35: 95-100, 1992.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Fibrillarin

Target: Fibrillarin (FBL)

Alternative Name: FBL

Background:

Synonyms: IFNGR2 protein, Interferon gamma receptor 2, IFNGR2, mCG_11456

Tissue Specificity: Wide tissue distribution (highest in the pancreas and very low in brain). Closely associated with the basal layer of cells in epithelia and the endothelium of blood vessel walls.

Background: rRNA 2'-O-methyltransferase fibrillarin is an enzyme that in humans is encoded by the FBL gene. This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8 % of humans with the autoimmune disease scleroderma recognize fibrillarin.

Molecular Weight: 37 kDa

Gene ID: 2091

UniProt: P22087

Pathways: Ribonucleoside Biosynthetic Process