Ancient Ubiquitous Protein 1 antibody (AA 77-399)

Catalog No. ABIN7602440

The Rabbit Polyclonal anti-Ancient Ubiquitous Protein 1 antibody (ABIN7602440) specifically detects Ancient Ubiquitous Protein 1 in WB, ELISA and FACS. The antibody is reactive with Human samples.

Quick Overview for Ancient Ubiquitous Protein 1 antibody (AA 77-399) (ABIN7602440)

Target: Ancient Ubiquitous Protein 1 (Aup1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ancient Ubiquitous Protein 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-Ancient Ubiquitous Protein 1 Antibody

Binding Specificity: AA 77-399

Purpose: Anti-AUP1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-AUP1 Antibody (ABIN7602440). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human AUP1 recombinant protein (Position: A77-R399).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Ancient Ubiquitous Protein 1

Target: Ancient Ubiquitous Protein 1 (Aup1)

Alternative Name: AUP1

Background:

Synonyms: DnaJ homolog subfamily A member 2, Cell cycle progression restoration gene 3 protein, Dnj3, Dj3, HIRA-interacting protein 4, Renal carcinoma antigen NY-REN-14, DNAJA2, CPR3, HIRIP4

Tissue Specificity: Expressed in fetal and adult brain. Also detected in fetal liver and skeletal muscle, but not in their adult counterparts.

Background: Ancient ubiquitous protein 1 is a protein that in humans is encoded by the AUP1 gene. The protein encoded this gene is involved in several pathways including quality control of misfolded proteins in the endoplasmic reticulum and lipid droplet accumulation. Lipid droplets are organelles in the cytoplasm that store neutral lipids such as cholesterol esters and trigylycerides to prevent the overabundance of free cholesterol and fatty acids in cells, but also to act as storage for other metabolic processes, such as membrane biogenesis. Reduced expression of this gene results in reduced lipid droplet clustering, a function that is dependent on ubiquitination of the protein. This protein contains multiple domains including a hydrophobic N-terminal domain, an acetyltranferase domain, a ubiquitin-binding CUE domain, and a UBE2B2-binding domain (G2BR). Alternative splicing results in multiple transcript variants.

Molecular Weight: 42 kDa

Gene ID: 550

UniProt: Q9Y679