ALKBH5 antibody (AA 81-180)

Catalog No. ABIN7602521

This anti-ALKBH5 antibody is a Rabbit Polyclonal antibody detecting ALKBH5 in WB, ELISA, ICC and IF. Suitable for Human.

Quick Overview for ALKBH5 antibody (AA 81-180) (ABIN7602521)

Target: ALKBH5 (AlkB, Alkylation Repair Homolog 5 (ALKBH5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALKBH5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-ALKBH5 Antibody

Binding Specificity: AA 81-180

Purpose: Anti-ALKBH5 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ALKBH5 Antibody Picoband® (ABIN7602521). Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ALKBH5 recombinant protein (Position: E81-R180).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Hartz, P. A. Personal Communication. Baltimore, Md. 3/12/2010. 2. Tsujikawa, K., Koike, K., Kitae, K., Shinkawa, A., Arima, H., Suzuki, T., Tsuchiya, M., Makino, Y., Furukawa, T., Konishi, N., Yamamoto, H. Expression and sub-cellular localization of human ABH family molecules. J. Cell. Molec. Med. 11: 1105-1116, 2007. 3. Zheng, G., Dahl, J. A., Niu, Y., Fedorcsak, P., Huang, C.-M., Li, C. J., Vagbo, C. B., Shi, Y., Wang, W.-L., Song, S.-H., Lu, Z., Bosmans, R. P. G., and 16 others. ALKBH5 is a mammalian RNA demethylase that impacts RNA metabolism and mouse fertility. Molec. Cell 49: 18-29, 2013.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ALKBH5

Target: ALKBH5 (AlkB, Alkylation Repair Homolog 5 (ALKBH5))

Alternative Name: ALKBH5

Background:

Synonyms: RNA demethylase ALKBH5, Alkylated DNA repair protein alkB homolog 5, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5, ALKBH5, ABH5, OFOXD1

Tissue Specificity: Widely expressed, with highest expression in lung, followed by testis, pancreas, spleen and ovary.

Background: RNA demethylase ALKBH5 is an enzyme that in humans is encoded by the ALKBH5 gene. It is mapped to 17p11.2. N (6)-methyladenosine (m (6)A) is the most prevalent modification of mRNA. ALKBH5 functions as a demethylase that removes m (6)A from mRNAs.

Molecular Weight: 52 kDa

Gene ID: 54890

UniProt: Q6P6C2