NEMF antibody (AA 81-607)

Catalog No. ABIN7602527

This anti-NEMF antibody is a Rabbit Polyclonal antibody detecting NEMF in WB, ELISA, ICC and IF. Suitable for Human.

Quick Overview for NEMF antibody (AA 81-607) (ABIN7602527)

Target: NEMF (SDCCAG1) (serologically Defined Colon Cancer Antigen 1 (SDCCAG1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEMF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-NEMF Antibody

Binding Specificity: AA 81-607

Purpose: Anti-SDCCAG1/NEMF Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SDCCAG1/NEMF Antibody Picoband® (ABIN7602527). Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SDCCAG1/NEMF recombinant protein (Position: H81-R607).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ahmed, A., Wang, M., Bergant, G., Maroofian, R., Zhao, R., Alfadhel, M., Nashabat, M., AlRifai, M. T., Eyaid, W., Alswaid, A., Beetz, C., Qin, Y., and 21 others. Biallelic loss-of-function variants in NEMF cause central nervous system impairment and axonal polyneuropathy. Hum. Genet. 140: 579-592, 2021. 2. Anazi, S., Maddirevula, S., Faqeih, E., Alsedairy, H., Alzahrani, F., Shamseldin, H. E., Patel, N., Hashem, M., Ibrahim, N., Abdulwahab, F., Ewida, N., Alsaif, H. S., and 36 others. Clinical genomics expands the morbid genome of intellectual disability and offers a high diagnostic yield. Molec. Psychiat. 22: 615-624, 2017. 3. Carbonnelle, D., Liehr, T., Jacquot, C., Masson, D., Lustenberger, P., Denis, M. G., Roussakis, C. Assignment of the serologically defined colon cancer antigen 1 gene (SDCCAG1) to human chromosome band 14q22 by in situ hybridization. Cytogenet. Cell Genet. 86: 248-249, 1999.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NEMF

Target: NEMF (SDCCAG1) (serologically Defined Colon Cancer Antigen 1 (SDCCAG1))

Alternative Name: NEMF

Background:

Synonyms: Tudor domain-containing protein 3, TDRD3

Tissue Specificity: Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Background: Nuclear export mediator factor (NEMF) is a protein that in humans is encoded by the NEMF gene. This gene encodes a component of the ribosome quality control complex. The encoded protein facilitates the recognition and ubiquitination of stalled 60S subunits by the ubiquitin ligase listerin. A similar protein in fly functions as a tumor suppressor.

Molecular Weight: 140 kDa

Gene ID: 9147

UniProt: O60524