NSUN7 antibody (AA 83-457)

Catalog No. ABIN7602547

The Rabbit Polyclonal anti-NSUN7 antibody has been validated for ELISA, WB and FACS. It is suitable to detect NSUN7 in samples from Human, Mouse and Rat.

Quick Overview for NSUN7 antibody (AA 83-457) (ABIN7602547)

Target: NSUN7 (NOP2/Sun Domain Family, Member 7 (NSUN7))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSUN7 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-NSUN7 Antibody

Binding Specificity: AA 83-457

Purpose: Anti-NSUN7 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-NSUN7 Antibody Picoband® (ABIN7602547). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NSUN7 recombinant protein (Position: D83-Q457). Human NSUN7 shares 79.2% amino acid (aa) sequence identity with mouse NSUN7.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL
1. Harris, T., Marquez, B., Suarez, S., Schimenti, J. Sperm motility defects and infertility in male mice with a mutation in Nsun7, a member of the Sun domain-containing family of putative RNA methyltransferases. Biol. Reprod. 77: 376-382, 2007. 2. Khosronezhad, N., Hosseinzadeh Colagar, A., Mortazavi, S. M. The Nsun7 (A11337)-deletion mutation, causes reduction of its protein rate and associated with sperm motility defect in infertile men. J. Assist. Reprod. Genet. 32: 807-815, 2015. 3. Ren, H. Y., Zhong, R., Ding, X. P., Chen, Z. Y., Jing, Y. L. Investigation of polymorphisms in exon7 of the NSUN7 gene among Chinese Han men with asthenospermia. Genet. Molec. Res. 14: 9261-9268, 2015.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NSUN7

Target: NSUN7 (NOP2/Sun Domain Family, Member 7 (NSUN7))

Alternative Name: NSUN7

Background:

Synonyms: NSUN7, Putative methyltransferase NSUN7, EC 2.1.1.-, NOL1/NOP2/Sun domain family member 7

Background: Predicted to enable RNA binding activity and methyltransferase activity. Predicted to be involved in RNA methylation. Predicted to act upstream of or within flagellated sperm motility and sperm mitochondrion organization.

Molecular Weight: 81 kDa

Gene ID: 79730