PYHIN1 antibody (AA 86-488)

Catalog No. ABIN7602574

The Rabbit Polyclonal anti-PYHIN1 antibody is suitable to detect PYHIN1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, FACS, IHC, ICC and IF.

Quick Overview for PYHIN1 antibody (AA 86-488) (ABIN7602574)

Target: PYHIN1 (Pyrin and HIN Domain Family, Member 1 (PYHIN1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PYHIN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-PYHIN1 Antibody

Binding Specificity: AA 86-488

Purpose: Anti-PYHIN1 Antibody

Characteristics: Anti-PYHIN1 Antibody (ABIN7602574). Tested in WB, IHC, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PYHIN1 recombinant protein (Position: K86-H488). Human PYHIN1 shares 45.6% amino acid (aa) sequence identity with mouse PYHIN1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry, 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PYHIN1

Target: PYHIN1 (Pyrin and HIN Domain Family, Member 1 (PYHIN1))

Alternative Name: PYHIN1

Background: The protein encoded by this gene belongs to the HIN-200 family of interferon-inducible proteins that share a 200-amino acid signature motif at their C-termini. HIN200 proteins are primarily nuclear and are involved in transcriptional regulation of genes important for cell cycle control, differentiation, and apoptosis. Downregulation of this gene is associated with breast cancer. This protein acts as a tumor suppressor by promoting ubiquitination and subsequent degradation of MDM2, which leads to stabilization of p53/TP53. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Molecular Weight: 55 kDa

Gene ID: 149628

UniProt: Q6K0P9