PC antibody (AA 88-1165)

Catalog No. ABIN7602587

The Rabbit Polyclonal anti-PC antibody (ABIN7602587) specifically detects PC in WB, IHC, ELISA, ICC, IF and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PC antibody (AA 88-1165) (ABIN7602587)

Target: PC (Pyruvate Carboxylase (PC))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-PC Antibody

Binding Specificity: AA 88-1165

Purpose: Anti-Pyruvate Carboxylase/PC Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human PC shares 97%,96.6% amino acid (aa) sequence identity with mouse,rat PC,respectively.

Characteristics: Anti-Pyruvate Carboxylase/PC Antibody. Tested in ELISA, IF, IHC, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Pyruvate Carboxylase/PC recombinant protein (Position: R88-D1165). Human PC shares 97% and 96.6% amino acid (aa) sequence identity with mouse and rat PC, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PC

Target: PC (Pyruvate Carboxylase (PC))

Alternative Name: Pyruvate Carboxylase

Background:

Background: This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene.

Gene Full Name: pyruvate carboxylase

Molecular Weight: 125 kDa

Gene ID: 5091

UniProt: P11498