IL-3 antibody (AA 91-166)

Catalog No. ABIN7602653

This anti-IL-3 antibody is a Rabbit Polyclonal antibody detecting IL-3 in WB, ELISA and IHC. Suitable for Mouse.

Quick Overview for IL-3 antibody (AA 91-166) (ABIN7602653)

Target: IL-3 (Interleukin 3 (IL-3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-IL-3 Antibody

Binding Specificity: AA 91-166

Purpose: Anti-IL3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-IL3 Antibody Picoband® (ABIN7602653). Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse IL3 recombinant protein (Position: E91-C166).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Yang YC, Ciarletta AB, Temple PA, Chung MP, Kovacic S, Witek-Giannotti JS, Leary AC, Kriz R, Donahue RE, Wong GG (October 1986). "Human IL-3 (multi-CSF): identification by expression cloning of a novel hematopoietic growth factor related to murine IL-3". Cell 47 (1): 3-10. 2. Webb, G. C., Lee, J. S., Campbell, H. D., Young, I. G. The genes for interleukins 3 and 5 map to the same locus on mouse chromosome 11. Cytogenet. Cell Genet. 50: 107-110, 1989.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.005 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for IL-3

Target: IL-3 (Interleukin 3 (IL-3))

Alternative Name: Il3

Background:

Synonyms: PH domain leucine-rich repeat-containing protein phosphatase 1, Pleckstrin homology domain-containing family E member 1, PH domain-containing family E member 1, Suprachiasmatic nucleus circadian oscillatory protein, hSCOP, PHLPP1, KIAA0606, PHLPP, PLEKHE1, SCOP

Tissue Specificity: Monocyte/macrophage specific.

Background: Interleukin 3, also known as IL-3, is a protein that in humans is encoded by the IL3 gene. It is mapped to 5q31.1. IL-3 is an interleukin, a type of biological signal (cytokine) that can improve the body's natural response to disease as part of the immune system. It acts by binding to the interleukin-3 receptor. IL-3 stimulates the differentiation of multipotent hematopoietic stem cells into myeloid progenitor cells or, with the addition of IL-7, into lymphoid progenitor cells. In addition, IL-3 stimulates proliferation of all cells in the myeloid lineage (granulocytes, monocytes, and dendritic cells), in conjunction with other cytokines, e.g., Erythropoietin (EPO), Granulocyte macrophage colony-stimulating factor (GM-CSF), and IL-6. IL-3 is secreted by basophils and activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response.

Molecular Weight: 26 kDa

Gene ID: 16187

UniProt: P01586

Pathways: JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy