SMC4 antibody (AA 911-1288)

Catalog No. ABIN7602662

This anti-SMC4 antibody is a Rabbit Polyclonal antibody detecting SMC4 in WB, ELISA, IF, ICC and FACS. Suitable for Human.

Quick Overview for SMC4 antibody (AA 911-1288) (ABIN7602662)

Target: SMC4 (Structural Maintenance of Chromosomes 4 (SMC4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMC4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-SMC4 Antibody

Binding Specificity: AA 911-1288

Purpose: Anti-SMC4 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SMC4 Antibody Picoband® (ABIN7602662). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SMC4 recombinant protein (Position: K911-C1288).

Isotype: IgG

Application Details

Application Notes: "Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
"1. Hirano T (2002). "The ABCs of SMC proteins: two-armed ATPases for chromosome condensation, cohesion, and repair". Genes Dev. 16 (4): 399-414. 2. Ball Jr AR, Yokomori K (2001). "The structural maintenance of chromosomes (SMC) family of proteins in mammals". Chromosome Res. 9 (2): 85-96. 3. Kimura K, Cuvier O, Hirano T (2001). "Chromosome condensation by a human condensin complex in Xenopus egg extracts". J. Biol. Chem. 276 (8): 5417-20.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for SMC4

Target: SMC4 (Structural Maintenance of Chromosomes 4 (SMC4))

Alternative Name: SMC4

Background:

Synonyms: Structural maintenance of chromosomes protein 4, SMC protein 4, SMC-4, Chromosome-associated polypeptide C, hCAP-C, XCAP-C homolog, SMC4, CAPC, SMC4L1

Tissue Specificity: Widely expressed. Higher expression in testis, colon, thymus.

Background: Structural maintenance of chromosomes protein 4 (SMC-4) also known as chromosome-associated polypeptide C (CAP-C) or XCAP-C homolog is a protein that in humans is encoded by the SMC4 gene. This gene belongs to the 'structural maintenance of chromosomes' (SMC) gene family. Members of this gene family play a role in two changes in chromosome structure during mitotic segregation of chromosomes- chromosome condensation and sister chromatid cohesion. The protein encoded by this gene is likely a subunit of the 13S condensin complex, which is involved in chromosome condensation. A pseudogene related to this gene is located on chromosome 2.

Molecular Weight: 180 kDa

Gene ID: 10051