Claudin 7 antibody (AA 92-211)

Catalog No. ABIN7602665

This anti-Claudin 7 antibody is a Rabbit Polyclonal antibody detecting Claudin 7 in WB, ELISA, IHC, FACS, IF and ICC. Suitable for Human.

Quick Overview for Claudin 7 antibody (AA 92-211) (ABIN7602665)

Target: Claudin 7 (CLDN7)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Claudin 7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-Claudin 7 Antibody

Binding Specificity: AA 92-211

Purpose: Anti-Claudin 7/CLDN-7 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Claudin 7/CLDN-7 Antibody Picoband® (ABIN7602665). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Claudin 7/CLDN-7 recombinant protein (Position: F92-V211).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 1-2 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Coyne, C. B., Gambling, T. M., Boucher, R. C., Carson, J. L., Johnson, L. G. Role of claudin interactions in airway tight junctional permeability. Am. J. Physiol. Lung Cell. Molec. Physiol. 285: L1166-L1178, 2003. 2. Kominsky, S. L., Argani, P., Korz, D., Evron, E., Raman, V., Garrett, E., Rein, A., Sauter, G., Kallioniemi, O.-P., Sukumar, S. Loss of the tight junction protein claudin-7 correlates with histological grade in both ductal carcinoma in situ and invasive ductal carcinoma of the breast. Oncogene 22: 2021-2033, 2003. 3. Morita, K., Furuse, M., Fujimoto, K., Tsukita, S. Claudin multigene family encoding four-transmembrane domain protein components of tight junction strands. Proc. Nat. Acad. Sci. 96: 511-516, 1999.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Claudin 7

Target: Claudin 7 (CLDN7)

Alternative Name: CLDN7

Background:

Synonyms: AF4/FMR2 family member 4,ALL1-fused gene from chromosome 5q31 protein,Protein AF-5q31,Major CDK9 elongation factor-associated protein,AFF4,AF5Q31, MCEF,HSPC092,

Tissue Specificity: Ubiquitously expressed. Strongly expressed in heart, placenta, skeletal muscle, pancreas and to a lower extent in brain. .

Background: Claudin-7 is a protein that in humans is encoded by the CLDN7 gene. This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is expressed constitutively in the mammary epithelium throughout development, and might be involved in vesicle trafficking to the basolateral membrane. It is essential for NaCl homeostasis in distal nephrons. The knockout mice lacking this gene showed severe salt wasting, chronic dehydration, and growth retardation, and died within 12 days after birth. Alternatively spliced transcript variants have been found for this gene.

Molecular Weight: 22 kDa

Gene ID: 1366

UniProt: O95471

Pathways: Hepatitis C