UNG antibody (AA 96-313)

Catalog No. ABIN7602695

This anti-UNG antibody is a Rabbit Polyclonal antibody detecting UNG in WB, ELISA, IHC, IF, ICC and FACS. Suitable for Human, Mouse, Rat and Monkey.

Quick Overview for UNG antibody (AA 96-313) (ABIN7602695)

Target: UNG (Uracil-DNA Glycosylase (UNG))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UNG antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-UNG Antibody

Binding Specificity: AA 96-313

Purpose: Anti-UNG Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-UNG Antibody Picoband® (ABIN7602695). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human UNG recombinant protein (Position: E96-L313).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat, Monkey
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Lindahl T, Ljungquist S, Siegert W, Nyberg B, Sperens B (May 1977). "DNA N-glycosidases: properties of uracil-DNA glycosidase from Escherichia coli". The Journal of Biological Chemistry. 252 (10): 3286-94. 2. Longo MC, Berninger MS, Hartley JL (Sep 1990). "Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions". Gene. 93 (1): 125-8. 3. Pearl LH (Aug 2000). "Structure and function in the uracil-DNA glycosylase superfamily". Mutation Research. 460 (3-4): 165-81.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for UNG

Target: UNG (Uracil-DNA Glycosylase (UNG))

Alternative Name: UNG

Background:

Synonyms: Phosphatidylethanolamine-binding protein 1, PEBP-1, HCNPpp, Neuropolypeptide h3, Prostatic-binding protein, Raf kinase inhibitor protein, RKIP, Hippocampal cholinergic neurostimulating peptide, HCNP, PEBP1, PBP, PEBP

Tissue Specificity: Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level).

Background: Uracil-DNA glycosylase, also known as UNG or UDG, is a human gene though orthologs exist ubiquitously among prokaryotes and eukaryotes and even in some DNA viruses. The first uracil DNA-glycosylase was isolated from Escherichia coli. This gene encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. Alternative promoter usage and splicing of this gene leads to two different isoforms: the mitochondrial UNG1 and the nuclear UNG2. The UNG2 term was used as a previous symbol for the CCNO gene, which has been confused with this gene, in the literature and some databases.

Molecular Weight: 35 kDa

Gene ID: 7374

UniProt: P13051

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response