NFKBIB antibody (AA 96-356)

Catalog No. ABIN7602697

The Rabbit Polyclonal anti-NFKBIB antibody has been validated for WB, ELISA and FACS. It is suitable to detect NFKBIB in samples from Human, Mouse and Rat.

Quick Overview for NFKBIB antibody (AA 96-356) (ABIN7602697)

Target: NFKBIB (NF-kappa-B inhibitor beta (NFKBIB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFKBIB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-NFKBIB Antibody

Binding Specificity: AA 96-356

Purpose: Anti-IkB Beta/NFKBIB Antibody Picoband®

Characteristics: Anti-IkB Beta/NFKBIB Antibody Picoband® (ABIN7602697). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human IkB Beta/NFKBIB recombinant protein (Position: T96-V356). Human NFKBIB shares 79.5% and 76.4% amino acid (aa) sequence identity with mouse and rat NFKBIB, respectively.

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Fenwick, C., S.-Y., Voll, R. E., Zhong, H., Im, S.-Y., Lee, J. W., Ghosh, S. A subclass of Ras proteins that regulate the degradation of I-kappa-B. Science 287: 869-873, 2000. 2. Hoffmann, A., Levchenko, A., Scott, M. L., Baltimore, D. The I-kappa-B-NF-kappa-B signaling module: temporal control and selective gene activation. Science 298: 1241-1245, 2002. Note: Erratum: Science 318: 1550 only, 2007. 3. Lee, J. W., Choi, H.-S., Gyuris, J., Brent, R., Moore, D. D. Two classes of proteins dependent on either the presence or absence of thyroid hormone for interaction with the thyroid hormone receptor. Molec. Endocr. 9: 243-254, 1995.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NFKBIB

Target: NFKBIB (NF-kappa-B inhibitor beta (NFKBIB))

Alternative Name: NFKBIB

Background: NF-kappa-B inhibitor beta is a protein that in humans is encoded by the NFKBIB gene. The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. Alternatively spliced transcript variants have been found for this gene.

Molecular Weight: 45 kDa

Gene ID: 4793

UniProt: Q15653

Pathways: NF-kappaB Signaling, Activation of Innate immune Response, Maintenance of Protein Location, Toll-Like Receptors Cascades