NG2 antibody (AA 962-1194)

Catalog No. ABIN7602702

This Rabbit Polyclonal antibody specifically detects NG2 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for NG2 antibody (AA 962-1194) (ABIN7602702)

Target: NG2 (MCSP) (Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NG2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-NG2 Antibody

Binding Specificity: AA 962-1194

Purpose: Anti-NG2/CSPG4 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NG2/CSPG4 Antibody Picoband® (ABIN7602702). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NG2/CSPG4 recombinant protein (Position: N962-D1194).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ligon, K. L., Kesari, S., Kitada, M., Sun, T., Arnett, H. A., Alberta, J. A., Anderson, D. J., Stiles, C. D., Rowitch, D. H. Development of NG2 neural progenitor cells requires Olig gene function. Proc. Nat. Acad. Sci. 103: 7853-7858, 2006. 2. Pluschke, G., Vanek, M., Evans, A., Dittmar, T., Schmid, P., Itin, P., Filardo, E. J., Reisfeld, R. A. Molecular cloning of a human melanoma-associated chondroitin sulfate proteoglycan. Proc. Nat. Acad. Sci. 93: 9710-9715, 1996. 3. Rettig, W. J., Dracopoli, N. C., Goetzger, T. A., Spengler, B. A., Biedler, J. L., Oettgen, H. F., Old, L. J. Somatic cell genetic analysis of human cell surface antigens: chromosomal assignments and regulation of expression in rodent-human hybrid cells. Proc. Nat. Acad. Sci. 81: 6437-6441, 1984.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for NG2

Target: NG2 (MCSP) (Melanoma Associated Chondroitin Sulfate Proteoglycan (MCSP))

Alternative Name: CSPG4

Background:

Synonyms: Vitamin D-binding protein, DBP, VDB, Gc-globulin, Group-specific component, Gc

Background: Chondroitin sulfate proteoglycan 4, also known as melanoma-associated chondroitin sulfate proteoglycan (MCSP) or neuron-glial antigen 2 (NG2), is a chondroitin sulfate proteoglycan that in humans is encoded by the CSPG4 gene. CSPG4 plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. It represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.

Molecular Weight: 310 kDa

Gene ID: 1464

Pathways: Glycosaminoglycan Metabolic Process