HSPA9 antibody (C-Term)

Catalog No. ABIN7602782

This anti-HSPA9 antibody is a Mouse Monoclonal antibody detecting HSPA9 in WB, IHC, IF, ICC and FACS. Suitable for Human, Rat and Mouse.

Quick Overview for HSPA9 antibody (C-Term) (ABIN7602782)

Target: HSPA9 (Heat Shock 70kDa Protein 9 (Mortalin) (HSPA9))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSPA9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 4I9

Product Details anti-HSPA9 Antibody

Binding Specificity: C-Term

Purpose: Anti-Grp75 Antibody Picoband® (monoclonal, 4I9)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Grp75 Antibody Picoband® (monoclonal, 4I9) (ABIN7602782). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Grp75, identical to the related mouse and rat sequences.

Isotype: IgG1

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Chen, T. H.-P., Kambal, A., Krysiak, K., Walshauser, M. A., Raju, G., Tibbitts, J. F., Walter, M. J. Knockdown of Hspa9, a del(5q31.2) gene, results in a decrease in hematopoietic progenitors in mice. Blood 117: 1530-1539, 2011. 2. Gross, M. B. Personal Communication. Baltimore, Md. 9/12/2011. 3. Kaul, S. C., Wadhwa, R., Matsuda, Y., Hensler, P. J., Pereira-Smith, O. M., Komatsu, Y., Mitsui, Y. Mouse and human chromosomal assignments of mortalin, a novel member of the murine hsp70 family of proteins. FEBS Lett. 361: 269-272, 1995.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for HSPA9

Target: HSPA9 (Heat Shock 70kDa Protein 9 (Mortalin) (HSPA9))

Alternative Name: HSPA9

Background:

Synonyms: CMT2L, CRYAC, DHMN 2, DHMN2, E2IG1, H11, HMN 2, HMN2, HMN2A, HSB8, HSPB 8, HspB8

Tissue Specificity: Predominantly expressed in skeletal muscle and heart.

Background: HSPA9 (heat shock 70 kDa protein 9 (mortalin)), also known as GRP75, mot-2, mthsp75, PBP74, HSPA9B, MORTALIN or MORTALIN, PERINUCLEAR, is a highly conserved member of the HSP70 family of proteins. It functions as a chaperone in the mitochondria, cytoplasm, and centrosome. The HSPA9 gene is mapped to chromosome 5q31.2 based on an alignment of the HSPA9 sequence with the genomic sequence. Knockdown of HSPA9 in erythroid cultures was associated with an increased number of cells in the G0/G1 phase of the cell cycle and accelerated apoptosis. Knockdown of Hspa9 in mouse bone marrow cells, followed by transplantation into wildtype recipients, also resulted in loss of erythroid cell number. Haploinsufficiency for HSPA9 may contribute to abnormal hematopoiesis in myelodysplastic syndromes. This protein plays a role in the control of cell proliferation.

Molecular Weight: 74 kDa

Gene ID: 3313

UniProt: P38646