ARL3 antibody (C-Term)

Catalog No. ABIN7602850

The Rabbit Polyclonal anti-ARL3 antibody has been validated for WB, IF, FACS and ICC. It is suitable to detect ARL3 in samples from Human, Mouse and Rat.

Quick Overview for ARL3 antibody (C-Term) (ABIN7602850)

Target: ARL3 (ADP-Ribosylation Factor-Like 3 (ARL3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARL3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-ARL3 Antibody

Binding Specificity: C-Term

Purpose: Anti-ARL3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-ARL3 Antibody Picoband® (ABIN7602850). Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human ARL3, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Alkanderi, S., Molinari, E., Shaheen, R., Elmaghloob, Y., Stephen, L. A., Sammut, V., Ramsbottom, S. A., Srivastava, S., Cairns, G., Edwards, N., Rice, S. J., Ewida, N., Alhashem, A., White, K., Miles, C. G., Steel, D. H., Alkuraya, F. S., Ismail, S., Sayer, J. A. ARL3 mutations cause Joubert syndrome by disrupting ciliary protein composition. Am. J. Hum. Genet. 103: 612-620, 2018. 2. Cavenagh, M. M., Breiner, M., Schurmann, A., Rosenwald, A. G., Terui, T., Zhang, C., Randazzo, P. A., Adams, M., Joost, H. G., Kahn, R. A. ADP-ribosylation factor (ARF)-like 3, a new member of the ARF family of GTP-binding proteins cloned from human and rat tissues. J. Biol. Chem. 269: 18937-18942, 1994. 3. Grayson, C., Bartolini, F., Chapple, J. P., Willison, K. R., Bhamidipati, A., Lewis, S. A., Luthert, P. J., Hardcastle, A. J., Cowan, N. J., Cheetham, M. E. Localization in the human retina of the X-linked retinitis pigmentosa protein RP2, its homologue cofactor C and the RP2 interacting protein Arl3. Hum. Molec. Genet. 11: 3065-3074, 2002.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ARL3

Target: ARL3 (ADP-Ribosylation Factor-Like 3 (ARL3))

Alternative Name: ARL3

Background:

Synonyms: ARL3, ARFL3, ADP-ribosylation factor-like protein 3

Background: ADP-ribosylation factor-like protein 3 is a protein that in humans is encoded by the ARL3 gene. Enables GDP binding activity, GTP binding activity, and microtubule binding activity. Involved in several processes, including cilium assembly, protein localization to cilium, and small GTPase mediated signal transduction. Acts upstream of or within post-Golgi vesicle-mediated transport. Located in several cellular components, including microtubule cytoskeleton, midbody, and photoreceptor connecting cilium. Implicated in Joubert syndrome and retinitis pigmentosa 83.

Molecular Weight: 23 kDa

Gene ID: 403

UniProt: P36405