DDC antibody (C-Term)

Catalog No. ABIN7602877

The Rabbit Polyclonal anti-DDC antibody has been validated for WB and FACS. It is suitable to detect DDC in samples from Human, Mouse and Rat.

Quick Overview for DDC antibody (C-Term) (ABIN7602877)

Target: DDC (Dopa Decarboxylase (Aromatic L-Amino Acid Decarboxylase) (DDC))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDC antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-DDC Antibody

Binding Specificity: C-Term

Purpose: Anti-DOPA decarboxylase/DDC Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-DOPA decarboxylase/DDC Antibody Picoband® (ABIN7602877). Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human DOPA decarboxylase/DDC, which shares 76% amino acid (aa) sequence identity with both mouse and rat DOPA decarboxylase/DDC.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Bonnin, A., Goeden, N., Chen, K., Wilson, M. L., King, J., Shih, J. C., Blakely, R. D., Deneris, E. S., Levitt, P. A transient placental source of serotonin for the fetal forebrain. Nature 472: 347-350, 2011. 2. Brun, L., Ngu, L. H., Keng, W. T., Ch'ng, G. S., Choy, Y. S., Hwu, W. L., Lee, W. T., Willemsen, M. A. A. P., Verbeek, M. M., Wassenberg, T., Regal, L., Orcesi, S., and 13 others. Clinical and biochemical features of aromatic L-amino acid decarboxylase deficiency. Neurology 75: 64-71, 2010. Note: Erratum: Neurology 75: 576 only, 2010. 3. Bruneau, G., Gross, M.-S., Krieger, M., Bernheim, A., Thibault, J., Nguyen, V. C. Preparation of a human DOPA decarboxylase cDNA probe by PCR and its assignment to chromosome 7. Ann. Genet. 33: 208-213, 1990.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for DDC

Target: DDC (Dopa Decarboxylase (Aromatic L-Amino Acid Decarboxylase) (DDC))

Alternative Name: DDC

Background:

Synonyms: DDC, AADC, Aromatic-L-amino-acid decarboxylase, AADC, EC 4.1.1.28, DOPA decarboxylase, DDC

Background: The encoded protein catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Defects in this gene are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD). AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene.

Molecular Weight: 50 kDa

Gene ID: 1644

UniProt: P20711

Pathways: Dopaminergic Neurogenesis