CXCR7 antibody (C-Term)

Catalog No. ABIN7602889

The Rabbit Polyclonal anti-CXCR7 antibody has been validated for WB, FACS and IHC. It is suitable to detect CXCR7 in samples from Human.

Quick Overview for CXCR7 antibody (C-Term) (ABIN7602889)

Target: CXCR7 (Chemokine (C-X-C Motif) Receptor 7 (CXCR7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCR7 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Product Details anti-CXCR7 Antibody

Binding Specificity: C-Term

Purpose: Anti-GPCR RDC1/CXCR-7/ACKR3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-GPCR RDC1/CXCR-7/ACKR3 Antibody Picoband® (ABIN7602889). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human GPCR RDC1/CXCR-7/ACKR3, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Berahovich, R. D., Zabel, B. A., Penfold, M. E. T., Lewen, S., Wang, Y., Miao, Z., Gan, L., Pereda, J., Dias, J., Slukvin, I. I., McGrath, K. E., Jaen, J. C., Schall, T. J. CXCR7 protein is not expressed on human or mouse leukocytes. J. Immun. 185: 5130-5139, 2010. 2. Boldajipour, B., Mahabaleshwar, H., Kardash, E., Reichman-Fried, M., Blaser, H., Minina, S., Wilson, D., Xu, Q., Raz, E. Control of chemokine-guided cell migration by ligand sequestration. Cell 132: 463-473, 2008. 3. Burns, J. M., Summers, B. C., Wang, Y., Melikian, A., Berahovich, R., Miao, Z., Penfold, M. E. T., Sunshine, M. J., Littman, D. R., Kuo, C. J., Wei, K., McMaster, B. E., Wright, K., Howard, M. C., Schall, T. J. A novel chemokine receptor for SDF-1 and I-TAC involved in cell survival, cell adhesion, and tumor development. J. Exp. Med. 203: 2201-2213, 2006.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CXCR7

Target: CXCR7 (Chemokine (C-X-C Motif) Receptor 7 (CXCR7))

Alternative Name: ACKR3

Background:

Synonyms: Dermatopontin, Tyrosine-rich acidic matrix protein, TRAMP, DPT

Tissue Specificity: Expressed in fibroblasts, heart, skeletal muscle, brain and pancreas. Expressed at an intermediate level in lung and kidney, and at a low level in liver and placenta. Expressed at a lower level in fibroblasts from hypertrophic scar lesional skin and in fibroblasts from patients with systemic sclerosis than in normal skin fibroblasts.

Background: Atypical chemokine receptor 3 also known as C-X-C chemokine receptor type 7 (CXCR-7) and G-protein coupled receptor 159 (GPR159) is a protein that in humans is encoded by the ACKR3 gene. This gene encodes a member of the G-protein coupled receptor family. Although this protein was earlier thought to be a receptor for vasoactive intestinal peptide (VIP), it is now considered to be an orphan receptor, in that its endogenous ligand has not been identified. The protein is also a coreceptor for human immunodeficiency viruses (HIV). Translocations involving this gene and HMGA2 on chromosome 12 have been observed in lipomas.

Molecular Weight: 41 kDa

Gene ID: 57007

UniProt: P25106

Pathways: Myometrial Relaxation and Contraction, Negative Regulation of intrinsic apoptotic Signaling