Hemoglobin Alpha 1 + 2 (HBA1,HBA2) (C-Term) antibody

Catalog No. ABIN7602893

The Rabbit Polyclonal anti-Hemoglobin Alpha 1 + 2 antibody (ABIN7602893) specifically detects Hemoglobin Alpha 1 + 2 in WB and IHC. The antibody is reactive with Human and Mouse samples.

Quick Overview for Hemoglobin Alpha 1 + 2 (HBA1,HBA2) (C-Term) antibody (ABIN7602893)

Target: Hemoglobin Alpha 1 + 2 (HBA1,HBA2)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details

Binding Specificity: C-Term

Purpose: Anti-Hemoglobin/HBA1/HBA2 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: identical to the related mouse,rat sequences.

Characteristics: Anti-Hemoglobin/HBA1/HBA2 Antibody. Tested in IHC, WB applications. This antibody reacts with Human, Mouse.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Hemoglobin/HBA1/HBA2, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse Immunohistochemistry(Paraffin-embedded Section), 1-2 μg/mL, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details

Target: Hemoglobin Alpha 1 + 2 (HBA1,HBA2)

Alternative Name: HBA1/HBA2

Background:

Background: The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97 % of the total hemoglobin, alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3 % of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1, some nondeletion alpha thalassemias have also been reported.

Gene Full Name: hemoglobin subunit alpha 1

Molecular Weight: 15 kDa

Gene ID: 3039, 3040

UniProt: P69905