MYL3/CMLC1 antibody (C-Term)

Catalog No. ABIN7602904

The Rabbit Polyclonal anti-MYL3/CMLC1 antibody has been validated for WB. It is suitable to detect MYL3/CMLC1 in samples from Human, Mouse and Rat.

Quick Overview for MYL3/CMLC1 antibody (C-Term) (ABIN7602904)

Target: MYL3/CMLC1 (MYL3) (Myosin, Light Chain 3 (MYL3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYL3/CMLC1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MYL3/CMLC1 Antibody

Binding Specificity: C-Term

Purpose: Anti-MYL3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-MYL3 Antibody Picoband® (ABIN7602904). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MYL3, which shares 91.3% amino acid (aa) sequence identity with both mouse and rat MYL3.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
1. Arad, M., Penas-Lado, M., Monserrat, L., Maron, B. J., Sherrid, M., Ho, C. Y., Barr, S., Karim, A., Olson, T. M., Kamisago, M., Seidman, J. G., Seidman, C. E. Gene mutations in apical hypertrophic cardiomyopathy. Circulation 112: 2805-2811, 2005. 2. Caleshu, C., Sakhuja, R., Nussbaum, R. L., Schiller, N. B., Ursell, P. C., Eng, C., De Marco, T., McGlothlin, D., Burchard, E. G., Rame, J. E. Furthering the link between the sarcomere and primary cardiomyopathies: restrictive cardiomyopathy associated with multiple mutations in genes previously associated with hypertrophic or dilated cardiomyopathy. Am. J. Med. Genet. 155A: 2229-2235, 2011. 3. Cohen-Haguenauer, O., Barton, P. J. R., Van Cong, N., Cohen, A., Masset, M., Buckingham, M., Frezal, J. Chromosomal assignment of two myosin alkali light-chain genes encoding the ventricular/slow skeletal muscle isoform and the atrial/fetal muscle isoform (MYL3, MYL4). Hum. Genet. 81: 278-282, 1989.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for MYL3/CMLC1

Target: MYL3/CMLC1 (MYL3) (Myosin, Light Chain 3 (MYL3))

Alternative Name: MYL3

Background:

Synonyms: MYL3, Myosin light chain 3, Cardiac myosin light chain 1, CMLC1, Myosin light chain 1, slow-twitch muscle B/ventricular isoform, MLC1SB, Ventricular myosin alkali light chain, Ventricular myosin light chain 1, VLCl, Ventricular/slow twitch myosin alkali light chain, MLC-lV/sb

Background: Myosin essential light chain (ELC), ventricular/cardiac isoform is a protein that in humans is encoded by the MYL3 gene. MYL3 encodes myosin light chain 3, an alkali light chain also referred to in the literature as both the ventricular isoform and the slow skeletal muscle isoform. Mutations in MYL3 have been identified as a cause of mid-left ventricular chamber type hypertrophic cardiomyopathy.

Molecular Weight: 25 kDa

Gene ID: 4634

UniProt: P08590