MYL3/CMLC1 antibody

Catalog No. ABIN969303

Cited in 2+ publications. The Mouse Monoclonal anti-MYL3/CMLC1 antibody (Clone 3F8) (ABIN969303) specifically detects MYL3/CMLC1 in WB, IHC and ELISA. The antibody is reactive with Human samples.

Quick Overview for MYL3/CMLC1 antibody (ABIN969303)

Target: MYL3/CMLC1 (MYL3) (Myosin, Light Chain 3 (MYL3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MYL3/CMLC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 3F8

Product Details anti-MYL3/CMLC1 Antibody

Purpose: MYL3 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of MYL3 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/10000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-MYL3/CMLC1 antibody (ABIN969303)

Haase, Dobbernack, Tünnemann, Karczewski, Cardoso, Petzhold, Schlegel, Lutter, Pierschalek, Behlke, Morano: "Minigenes encoding N-terminal domains of human cardiac myosin light chain-1 improve heart function of transgenic rats." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 7, pp. 865-73, (2006) (PubMed).

Xie, Huang, Huang, Zhou, Gong: "The functional domains of human ventricular myosin light chain 1." in: Biophysical chemistry, Vol. 106, Issue 1, pp. 57-66, (2003) (PubMed).

Target Details for MYL3/CMLC1

Target: MYL3/CMLC1 (MYL3) (Myosin, Light Chain 3 (MYL3))

Alternative Name: MYL3

Background: Myosins are a large superfamily of motor proteins that move along actin filaments, while hydrolyzing ATP. Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains and four light chains. Activation of smooth and cardiac muscle primarily involves pathways which increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. MYL3 encodes myosin light chain 3, an alkali light chain also referred to in the literature as both the ventricular isoform and the slow skeletal muscle isoform. Human myosin light chain has clinical application as a cardiac marker. Mutations in MYL3 have been identified as a cause of mid-left ventricular chamber type hypertrophic cardiomyopathy.

Molecular Weight: 22 kDa

Gene ID: 4634

UniProt: P08590