POGLUT1 antibody (C-Term)

Catalog No. ABIN7602910

The Rabbit Polyclonal anti-POGLUT1 antibody has been validated for WB, IHC, IF and ICC. It is suitable to detect POGLUT1 in samples from Human and Mouse.

Quick Overview for POGLUT1 antibody (C-Term) (ABIN7602910)

Target: POGLUT1 (Protein O-Glucosyltransferase 1 (POGLUT1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POGLUT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-POGLUT1 Antibody

Binding Specificity: C-Term

Purpose: Anti-POGLUT1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-POGLUT1 Antibody Picoband® (ABIN7602910). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at C-terminus of human POGLUT1, which shares 92.6% and 88.9% amino acid (aa) sequence identity with mouse and rat POGLUT1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
1. Basmanav, F. B., Oprisoreanu, A.-M., Pasternack, S. M., Thiele, H., Fritz, G., Wenzel, J., Grosser, L., Wehner, M., Wolf, S., Fagerberg, C., Bygum, A., Altmuller, J., and 9 others. Mutations in POGLUT1, encoding protein O-glucosyltransferase 1, cause autosomal-dominant Dowling-Degos disease. Am. J. Hum. Genet. 94: 135-143, 2014. 2. Chu, Q., Liu, L., Wang, W. Overexpression of hCLP46 enhances Notch activation and regulates cell proliferation in a cell type-dependent manner. Cell Prolif. 46: 254-262, 2013. 3. Hanneken, S., Rutten, A., Eigelshoven, S., Braun-Falco, M., Pasternack, S. M., Ruzicka, T., Nothen, M. M., Betz, R. C., Kruse, R. Morbus Galli-Galli: klinische und histopathologische Untersuchung anhand einer Fallserie von 18 Patienten. Hautarzt 62: 842-851, 2011.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for POGLUT1

Target: POGLUT1 (Protein O-Glucosyltransferase 1 (POGLUT1))

Alternative Name: POGLUT1

Background:

Synonyms: Intraflagellar transport protein 88 homolog, Recessive polycystic kidney disease protein Tg737 homolog, Tetratricopeptide repeat protein 10, TPR repeat protein 10, IFT88, TG737, TTC10

Tissue Specificity: Expressed in the heart, brain, liver, lung, kidney, skeletal muscle and pancreas.

Background: This gene encodes a protein with both O-glucosyltransferase and O-xylosyltransferase activity which localizes to the lumen of the endoplasmic reticulum. This protein has a carboxy-terminal KTEL motif which is predicted to function as an endoplasmic reticulum retention signal. This gene is an essential regulator of Notch signalling and likely plays a role in cell fate and tissue formation during development. It may also play a role in the pathogenesis of leukemia. Mutations in this gene have been associated with the autosomal dominant genodermatosis Dowling-Degos disease 4. Alternative splicing results in multiple transcript variants.

Molecular Weight: 55 kDa

Gene ID: 56983

Pathways: Notch Signaling