TLR9 antibody (C-Term)

Catalog No. ABIN7602935

This anti-TLR9 antibody is a Rabbit Polyclonal antibody detecting TLR9 in WB and IHC. Suitable for Human and Rat.

Quick Overview for TLR9 antibody (C-Term) (ABIN7602935)

Target: TLR9 (Toll-Like Receptor 9 (TLR9))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLR9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TLR9 Antibody

Binding Specificity: C-Term

Purpose: Anti-TLR9 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TLR9 Antibody Picoband® (ABIN7602935). Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human TLR9, which shares 97.1% amino acid (aa) sequence identity with mouse TLR9.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
1. Asagiri, M., Hirai, T., Kunigami, T., Kamano, S., Gober, H.-J., Okamoto, K., Nishikawa, K., Latz, E., Golenbock, D. T., Aoki, K., Ohya, K., Imai, Y., Morishita, Y., Miyazono, K., Kato, S., Saftig, P., Takayanagi, H. Cathepsin K-dependent Toll-like receptor 9 signaling revealed in experimental arthritis. Science 319: 624-627, 2008. 2. Babiuk, S., Mookherjee, N., Pontarollo, R., Griebel, P., van Drunen Littel-van den Hurk, S., Hecker, R., Babiuk, L. TLR9-/- and TLR9+/+ mice display similar immune responses to a DNA vaccine. Immunology 113: 114-120, 2004. 3. Baumann, C. L., Aspalter, I. M., Sharif, O., Pichlmair, A., Bluml, S., Grebien, F., Bruckner, M., Pasierbek, P., Aumayr, K., Planyavsky, M., Bennett, K. L., Colinge, J., Knapp, S., Superti-Furga, G. CD14 is a coreceptor of Toll-like receptors 7 and 9. J. Exp. Med. 207: 2689-2701, 2010.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TLR9

Target: TLR9 (Toll-Like Receptor 9 (TLR9))

Alternative Name: TLR9

Background:

Synonyms: Toll-like receptor 3, CD283, TLR3

Tissue Specificity: Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons.

Background: Toll-like receptor 9 is a protein that in humans is encoded by the TLR9 gene. The protein encoded by this gene is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Studies in mice and human indicate that this receptor mediates cellular response to unmethylated CpG dinucleotides in bacterial DNA to mount an innate immune response.

Molecular Weight: 116 kDa

Gene ID: 54106

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades