ATG4A antibody (C-Term)

Catalog No. ABIN7602947

This anti-ATG4A antibody is a Rabbit Monoclonal antibody detecting ATG4A in WB, IHC and IP. Suitable for Human, Mouse and Rat.

Quick Overview for ATG4A antibody (C-Term) (ABIN7602947)

Target: ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ATG4A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: ACHB-1

Product Details anti-ATG4A Antibody

Binding Specificity: C-Term

Purpose: Anti-Atg4A Monoclonal Antibody

Characteristics: Anti-Atg4A Monoclonal Antibody (ABIN7602947). Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Atg4A The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. Atg4 primes the Atg8 homologue for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7. The Atg8 homologue is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate. During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE, thereby recycling the Atg8 homologue.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for ATG4A

Target: ATG4A (Autophagy related 4A Cysteine Peptidase (ATG4A))

Alternative Name: ATG4A

Background:

Synonyms: Death domain-containing protein CRADD,Caspase and RIP adapter with death domain,RIP-associated protein with a death domain,CRADD,RAIDD,

Tissue Specificity: Constitutively expressed in most tissues, with particularly high expression in adult heart, testis, liver, skeletal muscle, fetal liver and kidney.

Molecular Weight: 45 kDa

Pathways: Autophagy