Notch1 antibody (Intracellular)

Catalog No. ABIN7602959

This anti-Notch1 antibody is a Rabbit Monoclonal antibody detecting Notch1 in WB, IHC, FACS, IF and ICC. Suitable for Human, Mouse and Rat.

Quick Overview for Notch1 antibody (Intracellular) (ABIN7602959)

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Notch1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: BGA-14

Product Details anti-Notch1 Antibody

Binding Specificity: Intracellular

Purpose: Anti-Notch1 Monoclonal Antibody

Characteristics: Anti-Notch1 Monoclonal Antibody (ABIN7602959). Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Notch1 Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis, negatively regulates endothelial cell proliferation and migration and angiogenic sprouting.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:3000
IHC 1:50-1:200
ICC/IF 1:100-1:500
FC 1:200-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for Notch1

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Alternative Name: NOTCH1

Background:

Synonyms: Signal transducer and activator of transcription 3,Acute-phase response factor,STAT3,APRF,

Tissue Specificity: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Molecular Weight: 110 kDa

UniProt: P46531

Pathways: Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development