ZAP70 antibody (Middle Region)

Catalog No. ABIN7602981

This Mouse Monoclonal antibody specifically detects ZAP70 in WB and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ZAP70 antibody (Middle Region) (ABIN7602981)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ZAP70 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: 9D5

Product Details anti-ZAP70 Antibody

Binding Specificity: Middle Region

Purpose: Anti-ZAP70 Antibody Picoband® (monoclonal, 9D5)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ZAP70 Antibody Picoband® (monoclonal, 9D5) (ABIN7602981). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human ZAP70, different from the related mouse sequence by two amino acids.

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Lupher ML, Reedquist KA, Miyake S, Langdon WY, Band H (Sep 1996). "A novel phosphotyrosine-binding domain in the N-terminal transforming region of Cbl interacts ly and selectively with ZAP-70 in T cells".The Journal of Biological Chemistry 271 (39): 24063-8. 2. Meng W, Sawasdikosol S, Burakoff SJ, Eck MJ (Mar 1999). "Structure of the amino-terminal domain of Cbl complexed to its binding site on ZAP-70 kinase". Nature 398 (6722): 84-90. 3. Neumeister EN, Zhu Y, Richard S, Terhorst C, Chan AC, Shaw AS (Jun 1995). "Binding of ZAP-70 to phosphorylated T-cell receptor zeta and eta enhances its autophosphorylation and generates specific binding sites for SH2 domain-containing proteins". Molecular and Cellular Biology 15 (6): 3171-8.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Alternative Name: ZAP70

Background:

Synonyms: SRK, STD, TZK, ZAP70, ZAP-70, ZAP 70, P43403

Tissue Specificity: Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells.

Background: ZAP-70 (Zeta-chain-associated protein kinase 70) encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.

Molecular Weight: 72 kDa

Gene ID: 7535

UniProt: P43403

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway