Annexin A3 antibody (Middle Region)

Catalog No. ABIN7602989

This anti-Annexin A3 antibody is a Mouse Monoclonal antibody detecting Annexin A3 in WB, IHC and FACS. Suitable for Human.

Quick Overview for Annexin A3 antibody (Middle Region) (ABIN7602989)

Target: Annexin A3 (ANXA3)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Annexin A3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 2H3H8

Product Details anti-Annexin A3 Antibody

Binding Specificity: Middle Region

Purpose: Anti-Annexin A3 Antibody Picoband® (monoclonal, 2H3H8)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Annexin A3 Antibody Picoband® (monoclonal, 2H3H8) (ABIN7602989). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Annexin A3, different from the related mouse sequence by one amino acid, and from the related rat sequence by three amino acids.

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Bianchi C, et al. Primary cell cultures from human renal cortex and renal-cell carcinoma evidence a differential expression of two spliced isoforms of Annexin A3. Am J Pathol, 2010 Apr. 2. Jung EJ, et al. Decreased annexin A3 expression correlates with tumor progression in papillary thyroid cancer. Proteomics Clin Appl, 2010 May. 3. Yan X, et al. Increased expression of annexin A3 is a mechanism of platinum resistance in ovarian cancer. Cancer Res, 2010 Feb 15.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for Annexin A3

Target: Annexin A3 (ANXA3)

Alternative Name: ANXA3

Background:

Synonyms: Cofilin-2, Cofilin, muscle isoform, CFL2

Tissue Specificity: Isoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues.

Background: Annexin A3 is a protein that in humans is encoded by the Annexin A3 gene. The Annexin A3 gene contains 13 exons and spans 58 kb of genomic DNA. The Annexin A3 gene is mapped to 4q21. It is abnormally expressed in fetuses of both IVF and ICSI, which may contribute to the increase risk of birth defects in these ART. This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions in the inhibition of phospholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. This protein may also play a role in anti-coagulation.

Molecular Weight: 36 kDa

Gene ID: 306

UniProt: P12429