ALOX5AP antibody (Middle Region)

Catalog No. ABIN7603016

Product Details anti-ALOX5AP Antibody

Target: ALOX5AP (Arachidonate 5-Lipoxygenase-Activating Protein (ALOX5AP))

Binding Specificity: Middle Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALOX5AP antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Purpose: Anti-ALOX5AP Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-ALOX5AP Antibody Picoband® (ABIN7603016). Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human ALOX5AP, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Dixon, R. A. F., Diehl, R. E., Opas, E., Rands, E., Vickers, P. J., Evans, J. F., Gillard, J. W., Miller, D. K. Requirement of a 5-lipoxygenase-activating protein for leukotriene synthesis. Nature 343: 282-284, 1990. 2. Ferguson, A. D., McKeever, B. M., Xu, S., Wisniewski, D., Miller, D. K., Yamin, T.-T., Spencer, R. H., Chu, L., Ujjainwalla, F., Cunningham, B. R., Evans, J. F., Becker, J. W. Crystal structure of inhibitor-bound human 5-lipoxygenase-activating protein. Science 317: 510-512, 2007. 3. Kennedy, B. P., Diehl, R. E., Boie, Y., Adam, M., Dixon, R. A. F. Gene characterization and promoter analysis of the human 5-lipoxygenase-activating protein (FLAP). J. Biol. Chem. 266: 8511-8516, 1991.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for ALOX5AP

Target: ALOX5AP (Arachidonate 5-Lipoxygenase-Activating Protein (ALOX5AP))

Alternative Name: ALOX5AP (ALOX5AP Products)

Background:

Synonyms: ALOX5AP, FLAP, Arachidonate 5-lipoxygenase-activating protein, FLAP, MK-886-binding protein

Background: This gene encodes a protein which, with 5-lipoxygenase, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.

Molecular Weight: 18 kDa

Gene ID: 241

UniProt: P20292