HSD17B3 antibody (Middle Region)

Catalog No. ABIN7603060

The Rabbit Polyclonal anti-HSD17B3 antibody is suitable to detect HSD17B3 in samples from Human, Rat and Mouse. It has been validated for WB and FACS.

Quick Overview for HSD17B3 antibody (Middle Region) (ABIN7603060)

Target: HSD17B3 (Hydroxysteroid (17-Beta) Dehydrogenase 3 (HSD17B3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSD17B3 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-HSD17B3 Antibody

Binding Specificity: Middle Region

Purpose: Anti-HSD17B3 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-HSD17B3 Antibody. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human HSD17B3, which shares 68.4% amino acid (aa) sequence identity with rat HSD17B3.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL/mL, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for HSD17B3

Target: HSD17B3 (Hydroxysteroid (17-Beta) Dehydrogenase 3 (HSD17B3))

Alternative Name: HSD17B3

Background:

Background: 17β-Hydroxysteroid dehydrogenase 3 (17β-HSD3) is an enzyme that in humans is encoded by the HSD17B3 gene and is involved in androgen steroidogenesis. This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia.

Gene Full Name: hydroxysteroid 17-beta dehydrogenase 3

Molecular Weight: 40 kDa

Gene ID: 3293

UniProt: P37058

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis