SOCS1 antibody (Middle Region)

Catalog No. ABIN7603094

This anti-SOCS1 antibody is a Rabbit Polyclonal antibody detecting SOCS1 in WB and IHC. Suitable for Human, Mouse and Rat.

Quick Overview for SOCS1 antibody (Middle Region) (ABIN7603094)

Target: SOCS1 (Suppressor of Cytokine Signaling 1 (SOCS1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOCS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SOCS1 Antibody

Binding Specificity: Middle Region

Purpose: Anti-SOCS1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SOCS1 Antibody Picoband® (ABIN7603094). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SOCS1, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
1. Ohya K, Kajigaya S, Yamashita Y, Miyazato A, Hatake K, Miura Y, Ikeda U, Shimada K, Ozawa K, Mano H.SOCS-1/JAB/SSI-1 can bind to and suppress Tec protein-tyrosine kinase. J Biol Chem. 1997 Oct 24, 272(43):27178-82. 2. Kramer JA, Adams MD, Singh GB, Doggett NA, Krawetz SA.A matrix associated region localizes the human SOCS-1 gene to chromosome 16p13.13. Somat Cell Mol Genet. 1998 Mar, 24(2):131-3. 3. Nagai H, Naka T, Terada Y, Komazaki T, Yabe A, Jin E, Kawanami O, Kishimoto T, Konishi N, Nakamura M, Kobayashi Y, Emi M.Hypermethylation associated with inactivation of the SOCS-1 gene, a JAK/STAT inhibitor, in human hepatoblastomas. J Hum Genet. 2003, 48(2):65-9. 4. Yoshida T, Ogata H, Kamio M, Joo A, Shiraishi H, Tokunaga Y, Sata M, Nagai H, Yoshimura A.SOCS1 is a suppressor of liver fibrosis and hepatitis-induced carcinogenesis. J Exp Med. 2004 Jun 21, 199(12):1701-7.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SOCS1

Target: SOCS1 (Suppressor of Cytokine Signaling 1 (SOCS1))

Alternative Name: SOCS1

Background:

Synonyms: RAF proto-oncogene serine/threonine-protein kinase,2.7.11.1,Proto-oncogene c-RAF,cRaf,Raf-1,RAF1,RAF,

Tissue Specificity: In skeletal muscle, isoform 1 is more abundant than isoform 2. .

Background: Suppressor of cytokine signaling 1(SOCS1), also known as Jak-binding protein(JAB), is a negative regulator to a subset of protein-tyrosine kinases. This 211-amino acid protein has a molecular mass of about 23.5 kD. The TEC-binding region of SOCS1 resides in the N terminus. SOCS1 associates with Tec and suppresses its kinase activity. The SOCS1 gene is intronless and is mapped to on chromosome 16p13.13. SOCS1 regulates the JAK/STAT signal-transduction pathway. Moreover, it contributes to protection against hepatic injury and fibrosis, and may also protect against hepatocarcinogenesis.

Molecular Weight: 27 kDa

Gene ID: 8651

UniProt: O15524

Pathways: JAK-STAT Signaling, Interferon-gamma Pathway, TLR Signaling, Response to Growth Hormone Stimulus