Vinexin antibody (N-Term)

Catalog No. ABIN7603121

This anti-Vinexin antibody is a Rabbit Polyclonal antibody detecting Vinexin in WB, IHC, IF, ICC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for Vinexin antibody (N-Term) (ABIN7603121)

Target: Vinexin (Sorbs3) (Sorbin and SH3 Domain Containing 3 (Sorbs3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Vinexin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-Vinexin Antibody

Binding Specificity: N-Term

Purpose: Anti-Vinexin/SORBS3 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Vinexin/SORBS3 Antibody Picoband® (ABIN7603121). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Vinexin/SORBS3, which shares 87.5% and 84.4% amino acid (aa) sequence identity with mouse and rat Vinexin/SORBS3, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Kioka, N., Sakata, S., Kawauchi, T., Amachi, T., Akiyama, S. K., Okazaki, K., Yaen, C., Yamada, K. M., Aota, S. Vinexin: a novel vinculin-binding protein with multiple SH3 domains enhances actin cytoskeletal organization. J. Cell Biol. 144: 59-69, 1999. 2. Mitsushima, M., Ueda, K., Kioka, N. Vinexin-beta regulates the phosphorylation of epidermal growth factor receptor on the cell surface. Genes Cells 11: 971-982, 2006. 3. Suwa, A., Mitsushima, M., Ito, T., Akamatsu, M., Ueda, K., Amachi, T., Kioka, N. Vinexin-beta regulates the anchorage dependence of ERK2 activation stimulated by epidermal growth factor. J. Biol. Chem. 277: 13053-13058, 2002.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Vinexin

Target: Vinexin (Sorbs3) (Sorbin and SH3 Domain Containing 3 (Sorbs3))

Alternative Name: SORBS3

Background:

Synonyms: Vinexin, SH3-containing adapter molecule 1, SCAM-1, Sorbin and SH3 domain-containing protein 3, SORBS3, SCAM1

Tissue Specificity: Both isoforms are expressed in different tissues like heart, placenta, brain, skeletal muscle and pancreas. Isoform beta is especially found in liver.

Background: Vinexin is a protein that in humans is encoded by the SORBS3 gene. It is mapped to 8p21.3. This gene encodes an SH3 domain-containing adaptor protein. The presence of SH3 domains play a role in this protein's ability to bind other cytoplasmic molecules and contribute to cystoskeletal organization, cell adhesion and migration, signaling, and gene expression. Multiple transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 87 kDa

Gene ID: 10174

UniProt: O60504

Pathways: MAPK Signaling