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HMGB3 antibody (N-Term)

The Mouse Monoclonal anti-HMGB3 antibody has been validated for WB, IHC, ICC, IF and FACS. It is suitable to detect HMGB3 in samples from Human.
Catalog No. ABIN7603126

Quick Overview for HMGB3 antibody (N-Term) (ABIN7603126)

Target

See all HMGB3 Antibodies
HMGB3 (High Mobility Group Box 3 (HMGB3))

Reactivity

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Human

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This HMGB3 antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone

7G13
  • Binding Specificity

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    N-Term

    Purpose

    Anti-HMG4 Antibody Picoband® (monoclonal, 7G13)

    Cross-Reactivity (Details)

    No cross-reactivity with other proteins.

    Characteristics

    Anti-HMG4 Antibody Picoband® (monoclonal, 7G13) (ABIN7603126). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Purification

    Immunogen affinity purified.

    Immunogen

    A synthetic peptide corresponding to a sequence at the N-terminus of human HMG4, identical to the related mouse and rat sequences.

    Isotype

    IgG2b
  • Application Notes

    Western blot, 0.1-0.5 μg/mL, Human
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human,
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    1. "Entrez Gene: HMGB3 high-mobility group box 3". 2. Davis DL, Burch JB (1992). "Isolation of a chicken HMG2 cDNA clone and evidence for an HMG2-specific 3'-untranslated region.". Gene 113 (2): 251-6. 3. Vaccari T, Beltrame M, Ferrari S, Bianchi ME (Aug 1998). "Hmg4, a new member of the Hmg1/2 gene family".Genomics 49 (2): 247-52.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Concentration

    500 μg/mL

    Buffer

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target

    HMGB3 (High Mobility Group Box 3 (HMGB3))

    Alternative Name

    HMG4

    Background

    Synonyms: "chromosomal protein, Nonhistone, HMG4 antibody|High mobility group (nonhistone chromosomal) protein 4 antibody|High mobility group box 3 antibody|High mobility group protein 2a antibody|High mobility group protein 4 antibody|High mobility group protein B3 antibody| High mobility group protein HMG4 antibody|HMG 4 antibody|HMG-2a antibody|HMG-4 antibody|HMG2A antibody|HMGB 3 antibody|HMGB3 antibody| HMGB3_HUMAN antibody|MGC90319 antibody|Non histone chromosomal protein antibody|Nonhistone chromosomal protein HMG4 antibody"

    Tissue Specificity: Expressed predominantly in placenta.

    Background: High-mobility group protein B, also known as HMG4, is a protein that in humans is encoded by the HMGB3 gene. This gene encodes a member of a family of proteins containing one or more high mobility group DNA-binding motifs. The encoded protein plays an important role in maintaining stem cell populations, and may be aberrantly expressed in tumor cells. A mutation in this gene was associated with microphthalmia, syndromic 13. There are numerous pseudogenes of this gene on multiple chromosomes. Alternative splicing results in multiple transcript variants.

    Molecular Weight

    23 kDa

    Gene ID

    3149

    UniProt

    O15347
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