Synaptophysin antibody (N-Term)

Catalog No. ABIN7603135

The Mouse Monoclonal anti-Synaptophysin antibody has been validated for WB. It is suitable to detect Synaptophysin in samples from Rat and Mouse.

Quick Overview for Synaptophysin antibody (N-Term) (ABIN7603135)

Target: Synaptophysin (SYP)

Reactivity: Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Synaptophysin antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 3G12

Product Details anti-Synaptophysin Antibody

Binding Specificity: N-Term

Purpose: Anti-SYP/Synaptophysin Antibody Picoband® (monoclonal, 3G12)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SYP/Synaptophysin Antibody Picoband® (monoclonal, 3G12) (ABIN7603135). Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Synaptophysin, identical to the related mouse and rat sequences.

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse, Rat1. Calhoun ME, Jucker M, Martin LJ, Thinakaran G, Price DL, Mouton PR (December 1996). "Comparative evaluation of synaptophysin-based methods for quantification of synapses". J. Neurocytol.25 (12): 821-8.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Synaptophysin

Target: Synaptophysin (SYP)

Alternative Name: SYP

Background:

Synonyms: MRXSYP|Major synaptic vesicle protein p38

Tissue Specificity: Isoform CFL2b is expressed predominantly in skeletal muscle and heart. Isoform CFL2a is expressed in various tissues.

Background: SYP (Synaptophysin) is located on the short arm of X chromosome (Xp11.23-p11.22). The protein is a synaptic vesicle glycoprotein with four transmembrane domains weighing 38 kDa. It is present inneuroendocrine cells and in virtually all neurons in the brain and spinal cord that participate in synaptic transmission. It acts as a marker for neuroendocrine tumors, and its ubiquity at the synapse has led to the use of synaptophysin immunostaining for quantification of synapses. Also, the protein binds cholesterol and is thought to targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR).

Molecular Weight: 38 kDa

Gene ID: 6855

UniProt: P08247, Q62277, P07825

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity