JunB antibody (N-Term)

Catalog No. ABIN7603223

This anti-JunB antibody is a Rabbit Polyclonal antibody detecting JunB in WB, IHC, IF, ICC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for JunB antibody (N-Term) (ABIN7603223)

Target: JunB (JUNB) (Jun B Proto-Oncogene (JUNB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JunB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-JunB Antibody

Binding Specificity: N-Term

Purpose: Anti-JUNB Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-JUNB Antibody (ABIN7603223). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human JUNB, which shares 88.9% amino acid (aa) sequence identity with both mouse and rat JUNB.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for JunB

Target: JunB (JUNB) (Jun B Proto-Oncogene (JUNB))

Alternative Name: JUNB

Background:

Synonyms: Thy-1 membrane glycoprotein, CDw90, Thy-1 antigen, CD90, THY1

Tissue Specificity: Detected adjacent to nodes of Ranvier in juxtaparanodal zones in spinal cord nerve fibers, but also in paranodal regions in some myelinated spinal cord axons (at protein level) (PubMed:11086297). Detected in the islet of Langerhans (PubMed:21483673).

Background: Transcription factor jun-B (JUNB) is a protein that in humans is encoded by the JUNB gene. It is mapped to 19p13.2. JUNB is a transcription factor involved in regulating gene activity following the primary growth factor response. It binds to the DNA sequence 5'-TGA[CG]TCA-3', and a large fraction (over 50 % ) of the JUNB locus is contained in these flanking evolutionarily conserved sequences (FECS), which may be required for effecting the proper transcriptional regulation of this gene. What's more, the expression of JUNB gene might be involved in terminal granulocyte differentiation or in regulating granulocyte functionality.

Molecular Weight: 42-45 kDa

Gene ID: 3726

UniProt: P17275