TPM2 antibody (N-Term)

Catalog No. ABIN7603245

This Rabbit Polyclonal antibody specifically detects TPM2 in WB, IHC, IF and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for TPM2 antibody (N-Term) (ABIN7603245)

Target: TPM2 (Tropomyosin-2 (TPM2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TPM2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-TPM2 Antibody

Binding Specificity: N-Term

Purpose: Anti-Tropomyosin 2/TPM2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Tropomyosin 2/TPM2 Antibody Picoband® (ABIN7603245). Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Tropomyosin 2/TPM2, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse, Rat
1. Bamshad, M., Watkins, W. S., Zenger, R. K., Bohnsack, J. F., Carey, J. C., Otterud, B., Krakowiak, P. A., Robertson, M., Jorde, L. B. A gene for distal arthrogryposis type I maps to the pericentromeric region of chromosome 9. Am. J. Hum. Genet. 55: 1153-1158, 1994. 2. Clarke, N. F., Domazetovska, A., Waddell, L., Kornberg, A., McLean, C., North, K. N. Cap disease due to mutation of the beta-tropomyosin gene (TPM2). Neuromusc. Disord. 19: 348-351, 2009. 3. Davidson, A. E., Siddiqui, F. M., Lopez, M. A., Lunt, P., Carlson, H. A., Moore, B. E., Love, S., Born, D. E., Roper, H., Majumdar, A., Jayadev, S., Underhill, H. R., and 14 others. Novel deletion of lysine 7 expands the clinical, histopathological and genetic spectrum of TPM2-related myopathies. Brain 136: 508-521, 2013.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TPM2

Target: TPM2 (Tropomyosin-2 (TPM2))

Alternative Name: TPM2

Background:

Synonyms: TNF receptor-associated factor 5,RING finger protein 84,TRAF5,RNF84,

Tissue Specificity: Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon, and peripheral blood.

Background: β-Tropomyosin, also known as tropomyosin beta chain is a protein that in humans is encoded by the TPM2 gene. This gene encodes beta-tropomyosin, a member of the actin filament binding protein family, and mainly expressed in slow, type 1 muscle fibers. Mutations in this gene can alter the expression of other sarcomeric tropomyosin proteins, and cause cap disease, nemaline myopathy and distal arthrogryposis syndromes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 38 kDa

Gene ID: 7169

UniProt: P07951