UCP3 antibody (N-Term)

Catalog No. ABIN7603247

The Rabbit Polyclonal anti-UCP3 antibody (ABIN7603247) specifically detects UCP3 in WB. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for UCP3 antibody (N-Term) (ABIN7603247)

Target: UCP3 (Uncoupling Protein 3 (Mitochondrial, Proton Carrier) (UCP3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCP3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-UCP3 Antibody

Binding Specificity: N-Term

Purpose: Anti-UCP3 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-UCP3 Antibody. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human UCP3, which shares 73.1% and 76.9% amino acid (aa) sequence identity with mouse and rat UCP3, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for UCP3

Target: UCP3 (Uncoupling Protein 3 (Mitochondrial, Proton Carrier) (UCP3))

Alternative Name: UCP3

Background:

Background: Mitochondrial uncoupling protein 3 is a protein that in humans is encoded by the UCP3 gene. Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. The different UCPs have tissue-specific expression, this gene is primarily expressed in skeletal muscle. This gene's protein product is postulated to protect mitochondria against lipid-induced oxidative stress. Expression levels of this gene increase when fatty acid supplies to mitochondria exceed their oxidation capacity and the protein enables the export of fatty acids from mitochondria. UCPs contain the three solcar protein domains typically found in MACPs. Two splice variants have been found for this gene.

Gene Full Name: uncoupling protein 3

Molecular Weight: 30 kDa

Gene ID: 7352

UniProt: P55916

Pathways: Proton Transport