His Tag antibody

Catalog No. ABIN7603839

This Mouse Monoclonal antibody specifically detects His Tag in WB, IP, IF and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for His Tag antibody (ABIN7603839)

Target: His Tag

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This His Tag antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 17H03

Product Details anti-His Tag Antibody

Purpose: Anti-His-Tag Mouse Monoclonal Antibody

Characteristics: Anti-His-Tag Mouse Monoclonal Antibody (ABIN7603839). Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide

Isotype: IgG1

Application Details

Application Notes: WB 1:1000-1:5000
ICC/IF 1:100-1:200
IP 1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Mouse IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for His Tag

Target: His Tag

Alternative Name: His-Tag

Target Type: Tag

Background:

Synonyms: Red fluorescent protein eqFP611,GFP-like chromoprotein,

Tissue Specificity: Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma. .

Molecular Weight: 50 kDa