CASP14 antibody

Catalog No. ABIN7604227

The Rabbit Monoclonal anti-CASP14 antibody is suitable to detect CASP14 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IP, ICC, IF and FACS.

Quick Overview for CASP14 antibody (ABIN7604227)

Target: CASP14 (Caspase 14, Apoptosis-Related Cysteine Peptidase (CASP14))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CASP14 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: ICI-3

Product Details anti-CASP14 Antibody

Purpose: Anti-Caspase-14 Rabbit Monoclonal Antibody

Characteristics: Anti-Caspase-14 Rabbit Monoclonal Antibody (ABIN7604227). Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human Caspase-14

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:20
FC 1:20

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: 0.5 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for CASP14

Target: CASP14 (Caspase 14, Apoptosis-Related Cysteine Peptidase (CASP14))

Alternative Name: CASP14

Background:

Synonyms: Caspase-14,CASP-14,3.4.22.-,Caspase-14 subunit p17, mature form,Caspase-14 subunit p10, mature form,Caspase-14 subunit p20, intermediate form,Caspase-14 subunit p8, intermediate form,CASP14,

Tissue Specificity: Expressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum) (at protein level). Expressed in keratinocytes of hair shaft and sebaceous glands (at protein level). In psoriatic skin only expressed at very low levels (PubMed:11175259). The p17/10 mature form is expressed in epidermis stratum corneum, the p20/p8 intermediate form in epidermis upper granular cells of the stratum granulosum (PubMed:22825846). .

Molecular Weight: 36 kDa

UniProt: P31944