MCAM antibody

Catalog No. ABIN7604288

This anti-MCAM antibody is a Rabbit Monoclonal antibody detecting MCAM in IHC, FACS, WB, IF, IP and ICC. Suitable for Human, Mouse and Rat.

Quick Overview for MCAM antibody (ABIN7604288)

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This MCAM antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: AbC83

Product Details anti-MCAM Antibody

Purpose: Anti-CD146 MCAM Rabbit Monoclonal Antibody

Characteristics: Anti- MCAM Rabbit Monoclonal Antibody (ABIN7604288). Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human CD146

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for MCAM

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Alternative Name: MCAM

Background:

Synonyms: Cell surface glycoprotein MUC18,Cell surface glycoprotein P1H12,Melanoma cell adhesion molecule,Melanoma-associated antigen A32,Melanoma-associated antigen MUC18,S-endo 1 endothelial-associated antigen,CD146,MCAM,MUC18,

Tissue Specificity: Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.

Molecular Weight: 120 kDa

UniProt: P43121