NAMPT antibody (AA 301-400) (HRP)

Catalog No. ABIN727109

The Rabbit Polyclonal anti-NAMPT antibody has been validated for WB, IHC (p) and IHC (fro). It is suitable to detect NAMPT in samples from Human, Rat and Mouse.

Quick Overview for NAMPT antibody (AA 301-400) (HRP) (ABIN727109)

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAMPT antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-NAMPT Antibody (HRP)

Binding Specificity: AA 301-400

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Cow,Pig,Horse,Chicken,Monkey

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PBEF

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NAMPT

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Alternative Name: PBEF1

Background:

Synonyms: VF, PBEF, PBEF1, VISFATIN, 1110035O14Rik, Nicotinamide phosphoribosyltransferase, NAmPRTase, Nampt, Pre-B-cell colony-enhancing factor 1, Pre-B cell-enhancing factor

Background: Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity).

Gene ID: 10135

UniProt: P43490