Dihydrofolate Reductase antibody

Catalog No. ABIN7604645

This anti-Dihydrofolate Reductase antibody is a Rabbit Monoclonal antibody detecting Dihydrofolate Reductase in WB, IHC, IF and ICC. Suitable for Human, Mouse and Rat.

Quick Overview for Dihydrofolate Reductase antibody (ABIN7604645)

Target: Dihydrofolate Reductase (DHFR)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Dihydrofolate Reductase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: ACBE-4

Product Details anti-Dihydrofolate Reductase Antibody

Purpose: Anti-DHFR Monoclonal Antibody

Characteristics: Anti-DHFR Monoclonal Antibody (ABIN7604645). Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human DHFR Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.

Isotype: IgG

Application Details

Application Notes: WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for Dihydrofolate Reductase

Target: Dihydrofolate Reductase (DHFR)

Alternative Name: DHFR

Background:

Synonyms: Scavenger receptor cysteine-rich type 1 protein M130,Hemoglobin scavenger receptor,CD163,Soluble CD163,sCD163,CD163,M130,

Tissue Specificity: Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood. .

Molecular Weight: 180 kDa

UniProt: P00374

Pathways: Mitotic G1-G1/S Phases