LIG4 antibody

Catalog No. ABIN7604658

This Rabbit Monoclonal antibody specifically detects LIG4 in WB, IHC, IF and ICC. It exhibits reactivity toward Human.

Quick Overview for LIG4 antibody (ABIN7604658)

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This LIG4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: ADHG-12

Product Details anti-LIG4 Antibody

Purpose: Anti-DNA Ligase IV Rabbit Monoclonal Antibody

Characteristics: Anti-DNA Ligase IV Rabbit Monoclonal Antibody (ABIN7604658). Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human DNA Ligase IV

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for LIG4

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Alternative Name: LIG4

Background:

Synonyms: Cell surface glycoprotein MUC18,Cell surface glycoprotein P1H12,Melanoma cell adhesion molecule,Melanoma-associated antigen A32,Melanoma-associated antigen MUC18,S-endo 1 endothelial-associated antigen,CD146,MCAM,MUC18,

Tissue Specificity: Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.

Molecular Weight: 120 kDa

UniProt: P49917

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response