E2F4 antibody

Catalog No. ABIN7604699

The Rabbit Monoclonal anti-E2F4 antibody (Clone AFDF-5) (ABIN7604699) specifically detects E2F4 in WB, IHC, IF, FACS and ICC. The antibody is reactive with Human samples.

Quick Overview for E2F4 antibody (ABIN7604699)

Target: E2F4 (E2F Transcription Factor 4, P107/p130-Binding (E2F4))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This E2F4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: AFDF-5

Product Details anti-E2F4 Antibody

Purpose: Anti-E2F4 Monoclonal Antibody

Characteristics: Anti-E2F4 Monoclonal Antibody (ABIN7604699). Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human E2F4 Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication.

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: 0.5 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for E2F4

Target: E2F4 (E2F Transcription Factor 4, P107/p130-Binding (E2F4))

Alternative Name: E2F4

Background:

Synonyms: Inhibitor of nuclear factor kappa-B kinase subunit alpha,I-kappa-B kinase alpha,IKK-A,IKK-alpha,IkBKA,IkappaB kinase,2.7.11.10,Conserved helix-loop-helix ubiquitous kinase,I-kappa-B kinase 1,IKK1,Nuclear factor NF-kappa-B inhibitor kinase alpha,NFKBIKA,Transcription factor 16,TCF-16,CHUK,IKKA, TCF16,

Tissue Specificity: Widely expressed.

Molecular Weight: 85 kDa

UniProt: Q16254

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size