GRP78 antibody

Catalog No. ABIN7605031

The Rabbit Monoclonal anti-GRP78 antibody is suitable to detect GRP78 in samples from Human, Rat and Mouse. It has been validated for WB, IHC, ICC and IF.

Quick Overview for GRP78 antibody (ABIN7605031)

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This GRP78 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Clone: BOO-8

Product Details anti-GRP78 Antibody

Purpose: Anti-GRP78 BiP HSPA5 Rabbit Monoclonal Antibody

Characteristics: Anti-GRP78 BiP HSPA5 Rabbit Monoclonal Antibody (ABIN7605031). Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human GRP78 BiP

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: 0.5 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for GRP78

Target: GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

Alternative Name: HSPA5

Background:

Synonyms: 78 kDa glucose-regulated protein,GRP-78,Endoplasmic reticulum lumenal Ca (2+)-binding protein grp78,Heat shock 70 kDa protein 5,Immunoglobulin heavy chain-binding protein,BiP,HSPA5,GRP78,

Tissue Specificity: Expressed in the excretory system, thyroid gland and Wilms tumors.

Molecular Weight: 28 kDa

UniProt: P11021

Pathways: Thyroid Hormone Synthesis, ER-Nucleus Signaling