HMGB1 antibody

Catalog No. ABIN7605135

This Rabbit Monoclonal antibody specifically detects HMGB1 in WB, IHC, IF, ICC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for HMGB1 antibody (ABIN7605135)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This HMGB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: AAC-8

Product Details anti-HMGB1 Antibody

Purpose: Anti-HMGB1/Hmg 1 Rabbit Monoclonal Antibody

Characteristics: Anti-HMGB1/Hmg 1 Rabbit Monoclonal Antibody (ABIN7605135). Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human HMGB1

Isotype: IgG

Application Details

Application Notes: WB 1:1000-1:2000
IHC 1:50-1:100
ICC/IF 1:50-1:100
FC 1:30

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: HMGB1

Background:

Synonyms: High mobility group protein B1,High mobility group protein 1,HMG-1,HMGB1,HMG1,

Tissue Specificity: Ubiquituous. Expressed in platelets (PubMed:11154118). .

Molecular Weight: 25 kDa

UniProt: P09429

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome