KDM1B antibody

Catalog No. ABIN7605454

The Rabbit Monoclonal anti-KDM1B antibody has been validated for WB, FACS, ICC, IP and IF. It is suitable to detect KDM1B in samples from Human, Mouse and Rat.

Quick Overview for KDM1B antibody (ABIN7605454)

Target: KDM1B (Lysine (K)-Specific Demethylase 1B (KDM1B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This KDM1B antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: ADAG-11

Product Details anti-KDM1B Antibody

Purpose: Anti-LSD2 / AOF1 Monoclonal Antibody

Characteristics: Anti-LSD2 / AOF1 Monoclonal Antibody (ABIN7605454). Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human LSD2 / AOF1 Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
ICC/IF 1:50-1:200
IP 1:60
FC 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for KDM1B

Target: KDM1B (Lysine (K)-Specific Demethylase 1B (KDM1B))

Alternative Name: KDM1B

Background:

Synonyms: Calponin-1,Basic calponin,Calponin H1, smooth muscle,CNN1,

Tissue Specificity: Smooth muscle, and tissues containing significant amounts of smooth muscle.

Molecular Weight: 93 kDa

Pathways: Warburg Effect