PARD6A antibody

Catalog No. ABIN7605853

The Rabbit Monoclonal anti-PARD6A antibody has been validated for WB and IP. It is suitable to detect PARD6A in samples from Human, Mouse and Rat.

Quick Overview for PARD6A antibody (ABIN7605853)

Target: PARD6A (Par-6 Partitioning Defective 6 Homolog alpha (PARD6A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PARD6A antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: AEDE-16

Product Details anti-PARD6A Antibody

Purpose: Anti-PAR6 Monoclonal Antibody

Characteristics: Anti-PAR6 Monoclonal Antibody (ABIN7605853). Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human PAR6 Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IP 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for PARD6A

Target: PARD6A (Par-6 Partitioning Defective 6 Homolog alpha (PARD6A))

Alternative Name: PARD6A

Background:

Synonyms: Laminin subunit alpha-3,Epiligrin 170 kDa subunit,E170,Epiligrin subunit alpha,Kalinin subunit alpha,Laminin-5 subunit alpha,Laminin-6 subunit alpha,Laminin-7 subunit alpha,Nicein subunit alpha,LAMA3,LAMNA,

Tissue Specificity: Skin, respiratory, urinary, and digestive epithelia and in other specialized tissues with prominent secretory or protective functions. Epithelial basement membrane, and epithelial cell tongue that migrates into a wound bed. A differential and focal expression of the subunit alpha-3 is observed in the CNS.

Molecular Weight: 50 kDa

Pathways: Cell-Cell Junction Organization