PRAS40 antibody

Catalog No. ABIN7606028

The Rabbit Monoclonal anti-PRAS40 antibody (Clone ACHD-1) (ABIN7606028) specifically detects PRAS40 in WB and IP. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PRAS40 antibody (ABIN7606028)

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PRAS40 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: ACHD-1

Product Details anti-PRAS40 Antibody

Purpose: Anti-PRAS40 AKT1S1 Monoclonal Antibody

Characteristics: Anti-PRAS40 AKT1S1 Monoclonal Antibody (ABIN7606028). Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human PRAS40 Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt. One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 protein. PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex.

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000
IP 1:20

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: 0.5 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for PRAS40

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Alternative Name: AKT1S1

Background:

Synonyms: Proline-rich AKT1 substrate 1,40 kDa proline-rich AKT substrate,AKT1S1 ,PRAS40 ,

Tissue Specificity: Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293). .

Molecular Weight: 58 kDa

UniProt: Q96B36

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Autophagy, BCR Signaling, Warburg Effect