PRAS40 antibody

Catalog No. ABIN7606029

This Rabbit Monoclonal antibody specifically detects PRAS40 in WB, IHC, IP and FACS. It exhibits reactivity toward Human and Mouse.

Quick Overview for PRAS40 antibody (ABIN7606029)

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PRAS40 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS)

Clone: ABIO-1

Product Details anti-PRAS40 Antibody

Purpose: Anti-PRAS40 AKT1S1 Rabbit Monoclonal Antibody

Characteristics: Anti-PRAS40 AKT1S1 Rabbit Monoclonal Antibody (ABIN7606029). Tested in WB, IHC, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Purification: Affinity-chromatography

Immunogen: A synthesized peptide derived from human PRAS40

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000
IHC 1:50-1:200
IP 1:50
FC 1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Concentration: Lot specific

Buffer: Rabbit IgG in phosphate buffered saline, pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol, 0.4-0.5 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Target Details for PRAS40

Target: PRAS40 (AKT1S1) (AKT1 Substrate 1 (Proline-Rich) (AKT1S1))

Alternative Name: AKT1S1

Background:

Synonyms: Proline-rich AKT1 substrate 1,40 kDa proline-rich AKT substrate,AKT1S1 ,PRAS40 ,

Tissue Specificity: Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A-549 and HeLa) than in normal cell lines (e.g. HEK293). .

Molecular Weight: 58 kDa

UniProt: Q96B36

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Cell Size, Autophagy, BCR Signaling, Warburg Effect